Effects of temperature and carbon source on the isotopic fractionations associated with O2 respiration for 17O/16O and 18O/16O ratios in E. coli. (1st November 2018)

• Record Type: Journal Article
• Authors: Stolper, Daniel A.; Fischer, Woodward W.; Bender, Michael L.
• Is Part Of: Geochimica et cosmochimica acta. Volume 240(2018)
• Page Start: 152
• Page End: 172
• Publication Date: 2018-11-01
• Subjects: Triple oxygen isotopes -- Aerobic respiration -- Dissolved oxygen; Geochemistry -- Periodicals; Meteorites -- Periodicals; Géochimie -- Périodiques; Météorites -- Périodiques; Geochemie; Astrochemie; Electronic journals; 551.905
• DOI: 10.1016/j.gca.2018.07.039
• Languages: English
• ISSNs: 0016-7037

Abstract

Abstract: 18 O/ 16 O and 17 O/ 16 O ratios of atmospheric and dissolved oceanic O2 are used as biogeochemical tracers of photosynthesis and respiration. Critical to this approach is a quantitative understanding of the isotopic fractionations associated with production, consumption, and transport of O2 in the ocean both at the surface and at depth. We made measurements of isotopic fractionations associated with O2 respiration by E. coli. Our study included wild-type strains and mutants with only a single respiratory O2 reductase in their electron transport chains (either a heme-copper oxygen reductase or a bd oxygen reductase). We tested two common assumptions made in interpretations of O2 isotope variations and in isotope-enabled models of the O2 cycle: ( i ) laboratory-measured respiratory 18 O/ 16 O isotopic fractionation factors ( 18 α) of microorganisms are independent of environmental and experimental conditions including temperature, carbon source, and growth rate; And ( ii ) the respiratory 'mass law' exponent, θ, between 18 O/ 16 O and 17 O/ 16 O, 17 α = ( 18 α) θ, is universal for aerobic respiration. Results demonstrated that experimental temperatures have an effect on both 18 α and θ for aerobic respiration. Specifically, lowering temperatures from 37 to 15 °C decreased the absolute magnitude of 18 α by 0.0025 (2.5‰), and caused the mass law slope to decrease by 0.005. We propose a possible biochemical basis for these variations using a model of O2 reduction that incorporates two isotopically discriminating steps: the reversible binding and unbinding of O2 to a terminal reductase, and the irreversible reduction of that O2 to water. Finally, we cast our results in a one-dimensional isopycnal reaction-advection-diffusion model, which demonstrates that enigmatic δ 18 O and Δ 17 O variations of dissolved O2 in the dark ocean can be understood by invoking the observed temperature dependence of these isotope effects.