Draft genome sequences of extended-spectrum β-lactamase-producing Enterobacter aerogenes isolated from swine and human. (September 2018)

• Record Type: Journal Article
• Authors: Founou, Luria Leslie; Founou, Raspail Carrel; Allam, Mushal; Ismail, Arshad; Essack, Sabiha Yusuf
• Is Part Of: Journal of global antimicrobial resistance. Volume 14(2018)
• Page Start: 70
• Page End: 71
• Publication Date: 2018-09
• Subjects: Enterobacter aerogenes -- Extended-spectrum β-lactamase -- ESBL -- Antibiotic resistance -- Whole-genome sequencing -- Food chain; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2018.06.006
• Languages: English
• ISSNs: 2213-7165

Abstract

Abstract: Objectives: The draft genome sequences of two Enterobacter aerogenes strains (HN503E2II and PN108E5IIB) isolated from two Cameroonian abattoirs are reported. Methods: Bacterial genomic DNA of the two isolates was sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using CLC Genomics Workbench and SPAdes. The assembled contigs were annotated, and antibiotic resistance genes, virulence factors and plasmids were identified. Results: Whole-genome sequencing revealed that both strains were similar, with genomes of 4 878 638 bp and 4 794 257 bp, encoding several resistance genes associated with resistance to β-lactams, fluoroquinolones, aminoglycosides, fosfomycin, phenicols, sulphonamides, trimethoprim, macrolides and tetracycline. In silico analysis also revealed chromosomal integration of one plasmid in the genome of PN108E5IIB. Conclusion: The genome sequences reported here will provide useful information for a better understanding of the genetic structure of E. aerogenes in Africa.