Anaerobic and sequential aerobic production of high-titer ethanol and single cell protein from NaOH-pretreated corn stover by a genome shuffling-modified Saccharomyces cerevisiae strain. (October 2016)

• Record Type: Journal Article
• Authors: Ren, Xueliang; Wang, Juncong; Yu, Hui; Peng, Chunlan; Hu, Jinlong; Ruan, Zhiyong; Zhao, Shumiao; Liang, Yunxiang; Peng, Nan
• Is Part Of: Bioresource technology. Volume 218(2016)
• Page Start: 623
• Page End: 630
• Publication Date: 2016-10
• Subjects: Ethanol fermentation -- Corn stover -- Single cell protein -- Genome shuffling -- Simultaneous saccharification and fermentation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.06.118
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: A genome shuffling-modified S. cerevisiae strain 14 was constructed. Strain 14 produced ethanol with a yield 0.47 g/g glucose at 35 °C. Strain 14 contained 564 mg/g crude protein and most essential amino acids. Strain 14 produced 64.07 g/L ethanol with the yield of 0.26 g/g stover. Abstract: In this study, a Saccharomyces cerevisiae recombinant strain 14 was constructed through genome shuffling method by transferring the whole genomic DNA of Candida intermedia strain 23 into a thermo-tolerant S. cerevisiae strain. The recombinant strain 14 combined the good natures of both parent strains that efficiently produced ethanol from glucose and single cell protein from xylose with 54.6% crude protein and all essential amino acids except cysteine at 35 °C. Importantly, the recombinant strain 14 produced 64.07 g/L ethanol from 25% (w/v) NaOH-pretreated and washed corn stover with the ethanol yield of 0.26 g/g total stover by fed-batch simultaneous saccharification and fermentation and produced 66.50 g/L dry cell mass subsequently from the residual hydrolysate and ethanol. Therefore, this study represents a feasible method to comprehensively utilize hexose and pentose in lignocellulosic materials.