Improvement of exopolysaccharide production by Porphyridium marinum. (August 2016)

• Record Type: Journal Article
• Authors: Soanen, Nastasia; Da Silva, Elise; Gardarin, Christine; Michaud, Philippe; Laroche, Céline
• Is Part Of: Bioresource technology. Volume 213(2016)
• Page Start: 231
• Page End: 238
• Publication Date: 2016-08
• Subjects: Porphyridium -- Polysaccharide -- Photobioreactor -- Semi-continuous culture -- Microalgae; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.02.075
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Porphyridium marinum is a source of sulfated exopolysaccharides. Stoechiometry of culture medium influenced the EPS production. Optimal conditions of irradiance, pH and temperature optimized EPS production. Semi-continuous culture increased EPS productivity. Abstract: With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205 S0.0597 P0.005 . Cultivation on this medium allowed the increase of EPS concentration up to 2.5 g L −1, without modification of the EPS productivity (0.096 g L −1 ) and EPS structure. In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production. A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360 μmol photons m −2 s −1 and 28 °C led to an EPS process productivity of 0.031 g h −1 instead of 0.020 g h −1 in batch culture.