Influence of two-phase anaerobic digestion on fate of selected antibiotic resistance genes and class I integrons in municipal wastewater sludge. (July 2016)

• Record Type: Journal Article
• Authors: Wu, Ying; Cui, Erping; Zuo, Yiru; Cheng, Weixiao; Rensing, Christopher; Chen, Hong
• Is Part Of: Bioresource technology. Volume 211(2016)
• Page Start: 414
• Page End: 421
• Publication Date: 2016-07
• Subjects: Two-phase anaerobic digestion -- Antibiotic resistance genes -- Bacterial community structure -- Bacterial pathogens; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.03.086
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: TAPR resulted in a remarkable reduction of most ARGs. Proteobacteria and Actinobacteria were the main bacterial phyla affecting the fate of ARGs. All reactors were effective in disinfecting bacterial pathogens harboring high-risk ARGs. Positive correlations were found between class I integrons and five types of ARGs. Abstract: The response of representative antibiotic resistance genes (ARGs) to lab-scale two-phase (acidogenic/methanogenic phase) anaerobic digestion processes under thermophilic and mesophilic conditions was explored. The associated microbial communities and bacterial pathogens were characterized by 16S rRNA gene sequencing. A two-phase thermophilic digestion reduced the presence of tetA, tetG, tetX, sul1, ermB, dfrA1, dfrA12 and intI1 exhibiting 0.1–0.72 log unit removal; in contrast, tetO, tetW, sul3, ermF and blaTEM even increased relative to the feed, and sul2 showed no significant decrease. The acidogenic phase of thermophilic digestion was primarily responsible for reducing the quantity of these genes, while the subsequent methanogenic phase caused a rebound in their quantity. In contrast, a two-phase mesophilic digestion process did not result in reducing the quantity of all ARGs and intI1 except for ermB and blaTEM . ARGs patterns were correlated with Proteobacteria and Actinobacteria during the two-phase anaerobic digestion.