Denitrifying phosphorus removal from municipal wastewater and dynamics of "Candidatus Accumulibacter" and denitrifying bacteria based on genes of ppk1, narG, nirS and nirK. (May 2016)

• Record Type: Journal Article
• Authors: Zeng, Wei; Zhang, Jie; Wang, Anqi; Peng, Yongzhen
• Is Part Of: Bioresource technology. Volume 207(2016)
• Page Start: 322
• Page End: 331
• Publication Date: 2016-05
• Subjects: Candidatus Accumulibacter -- Denitrifying phosphorus removal -- Municipal wastewater -- Polyphosphate kinase 1 gene (ppk1) -- Population dynamics; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.02.016
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Denitrifying P removal from real municipal wastewater was achieved, reaching 90%. Clades IIC and IIF were the dominant Accumulibacter during denitrifying P removal. NirS was the main functional gene to execute nitrite reduction. Denitrifying P removal changed clade-level community structure of Accumulibacter. Ratio of ( nirS + nirK )/ ppk 1 could estimate the ratio of denitrifying Accumulibacter. Abstract: Relevance of clade-level population dynamics of " Candidatus Accumulibacter" to performance of denitrifying phosphorus (P) removal from municipal wastewater was investigated. Stable denitrifying P removal in anoxic zone of continuous-flow reactor was achieved, accounting for 90% of total P removal. Clades IIC and IIF affiliated with Accumulibacter lineage were the dominant clades during denitrifying P removal, reaching 90% of ppk 1 clone library. NarG gene library indicated Gamma and Beta-proteobacteria played an important role in nitrate reduction. Diversity and abundance of nirS library was much more than nirK, and thus became the main functional gene to execute nitrite reduction. Based on abundance of nirS, nirK and ppk 1, the ratio of Accumulibacter capable of denitrifying P removal to total Accumulibacter was 22%. No matter whether Accumulibacter had narG gene or not, high abundance of narG at a level of 10 9 cells/(g dried-sludge) promoted nitrate reduced to nitrite, ensuring performance of denitrifying P removal.