Is the chronic impact of sulfamethoxazole different for slow growing culture? The effect of culture history. (April 2016)

• Record Type: Journal Article
• Authors: Kor-Bicakci, Gokce; Pala-Ozkok, Ilke; Ural, Aslihan; Jonas, Daniel; Orhon, Derin; Ubay-Cokgor, Emine
• Is Part Of: Bioresource technology. Volume 206(2016)
• Page Start: 65
• Page End: 76
• Publication Date: 2016-04
• Subjects: Biodegradation -- Inhibitory impact -- Sulfamethoxazole -- Process modeling -- 454-Pyrosequencing; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.01.069
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Acute impact of SMX boosted endogenous respiration due to higher cell maintenance. Chronic exposure to SMX resulted in retardation and reduction of substrate storage. Part of acetate is utilized at slower rate due to binding/release effect of SMX. Microbial population structure was assessed using sequencing of 16S rRNA gene. Amaricoccus and Bacteroidetes spp. proliferated owing to co-metabolism of SMX. Abstract: The study evaluated impact of sulfamethoxazole on acetate utilization kinetics and microbial community structure using respirometric analysis and pyrosequencing. A fill and draw reactor fed with acetate was sustained at a sludge age of 10 days. Acute impact was assessed by modeling of respirometric data in batch reactors started with sulfamethoxazole doses in the range of 25–200 mg/L. Fill and draw operation resumed with continuous sulfamethoxazole dosing of 50 mg/L and the chronic impact was evaluated with acclimated biomass after 20 days. Acute impact revealed higher maintenance energy requirements, activity reduction and slight substrate binding. Chronic impact resulted in retardation of substrate storage. A fraction of acetate was utilized at a much lower rate with partial biodegradation of sulfamethoxazole by the acclimated biomass. Pyrosequencing indicated that Amaricoccus sp. and an unclassified Bacteroidetes sp., possibly with the ability to co-metabolize sulfamethoxazole, dominated the community.