Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides. (April 2016)

• Record Type: Journal Article
• Authors: Huang, Xiang-Feng; Liu, Jia-Nan; Lu, Li-Jun; Peng, Kai-Ming; Yang, Gao-Xiang; Liu, Jia
• Is Part Of: Bioresource technology. Volume 206(2016)
• Page Start: 141
• Page End: 149
• Publication Date: 2016-04
• Subjects: Microbial oils -- Rhodosporidium toruloides -- Acetic acid -- Culture strategies; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.01.073
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: R. toruloides can use 4–20 g/L acetic acid to produce lipids. Sequencing batch process was developed for lipid production using low-content VFAs. Different performances were found between media containing acetic acid and glucose. The C/N affects acetic acid utilization ratio and lipid content simultaneously. Abstract: Rhodosporidium toruloides AS 2.1389 was tested using different concentrations of acetic acid as a low-cost carbon source for the production of microbial lipids, which are good raw materials for biodiesel production. It grew and had higher lipid contents in media containing 4–20 g/L acetic acid as the sole carbon source, compared with that in glucose-containing media under the same culture conditions. At acetic acid concentrations as high as 20 g/L and the optimal carbon-to-nitrogen ratio (C/N) of 200 in a batch culture, the highest biomass production was 4.35 g/L, with a lipid content of 48.2%. At acetic acid concentrations as low as 4 g/L, a sequencing batch culture (SBC) with a C/N of 100 increased biomass production to 4.21 g/L, with a lipid content of 38.6%. These results provide usable culture strategies for lipid production by R. toruloides AS 2.1389 when using diverse waste-derived volatile fatty acids.