Afamin promotes glucose metabolism in papillary thyroid carcinoma. (15th October 2016)

• Record Type: Journal Article
• Authors: Shen, Chen-Tian; Wei, Wei-Jun; Qiu, Zhong-Ling; Song, Hong-Jun; Luo, Quan-Yong
• Is Part Of: Molecular and cellular endocrinology. Volume 434(2016)
• Page Start: 108
• Page End: 115
• Publication Date: 2016-10-15
• Subjects: Afamin -- Thyroid carcinoma -- Glucose metabolism -- Gene expression -- 18F-FDG; AFM afamin -- DTC differentiated thyroid cancer -- RAI radioactive iodine -- TSH thyroid-stimulating hormone -- 18F-FDG 18F-fluoro-2-deoxy-D-glucose -- PET/CT positron emission tomography/computer tomography -- PTC papillary thyroid cancer -- OSEM3D three-dimensional ordered-subset expectation maximization -- SUVmax maximum standardized uptake value -- PARP1 Poly ADP-ribose polymerase-1; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2016.06.013
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Circulating afamin (AFM) concentrations have been investigated as a tumor biomarker in various types of carcinomas. However, suitable cell lines expressing human afamin have not yet been reported and current knowledge of the functions of afamin, particularly at the mechanistic molecular level, is very limited. In the current study, thyroid cancer cell lines 8505c and K1 were used to investigate the potential functions of afamin. AFM over-expression models and vector controls of 8505c (8505c + AFM and 8505c + NC) and K1 (K1 + AFM and K1 + NC) were successfully established by Lenti-LV5-AFM and Lenti-LV5-NC transfection. The change of gene expression was detected by qRT-PCR and western blotting analysis. 18 F-FDG imaging in xenografts model was performed using a micro PET/CT. We found that protein level of GAPDH, GLUT1, HK2, p-AKT, AKT, p-mTOR and PARP1 were up-regulated in K1 + AFM cells when compared to K1 and K1 + NC. While in 8505c, 8505c + NC and 8505c cells, the expression level of these genes were not significantly changed. 18 F-FDG uptake was much higher in K1 + AFM cells when compared to K1 and K1 + NC in vitro and in vivo. In conclusion, afamin could promote glycometabolism by up-regulating the glucose metabolism key enzymes in papillary thyroid carcinoma. These findings reveal new clues of the molecular function of AFM. Highlights: Afamin promotes glycometabolism of PTC in vitro and in vivo. Afamin increases glucose metabolism key enzymes expression in PTC. Afamin up-regulates the expression of PARP1 in PTC. AKT/mTOR pathway activation may take part in the process of this effect.