Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp. strain HC8-3S. (February 2016)

• Record Type: Journal Article
• Authors: Liu, Yuhua; Hu, Xiaoke; Liu, Hui
• Is Part Of: International biodeterioration & biodegradation. Volume 107(2016)
• Page Start: 56
• Page End: 61
• Publication Date: 2016-02
• Subjects: Acinetobacter sp. -- Industrial-scale fermentation -- Long-term storage -- Crude oil-degrading bacterium; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2015.11.007
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: The marine bacterium Acinetobacter sp. strain HC8-3S, capable of degrading petroleum hydrocarbons, was previously shown to be applicable for bioremediation. Here, we evaluated the performance of the strain during industrial-scale fermentation, long-term storage, and biodegradation processes. Fermentation parameters were optimized for industrial-scale culturing using lower salinity and fewer inorganic salts in the culture medium. The shelf-life of the fermentation broth was evaluated in different storage conditions. Biodegradation efficiency of the strain was evaluated using gas chromatography. Results indicated that the optimum conditions for one-ton scale fermentation were 30 °C, pH 7.4–7.8, and rotation speed 90 rpm; the cell density reached 3.6 ± 1.9 × 10 10 CFU ml −1 after 12 h. The low temperature can preserved fermentation broth for longer time. When the storage temperature was down to 4 °C from room temperature, half-life of strain HC8-3S extended from 9 days to 34 days. The biodegradation rate of the saturated hydrocarbon fraction of crude oil was 94% after treatment with the strain for 5 days. The results indicate that Acinetobacter sp. strain HC8-3S can be enriched efficiently on a large scale, making it a potentially useful industrial strain. This crude oil degradative capability of the strain HC8-3S provides possible application for the clean-up of crude oil-contaminated environment. Highlights: We cultured a marine crude oil-degrading bacterium on industrial scale. Fermentation conditions of Acinetobacter sp. strain HC8-3S were optimized. Shelf-life of fermentation broth was 120 d at 4 °C and 60 d at ambient temperature. The fermentation broth of HC8-3S can effectively degrade 94% crude oil in 5 days.