Changes in integrity of the gill during histidine deficiency or excess due to depression of cellular anti-oxidative ability, induction of apoptosis, inflammation and impair of cell-cell tight junctions related to Nrf2, TOR and NF-κB signaling in fish. (September 2016)

• Record Type: Journal Article
• Authors: Jiang, Wei-Dan; Feng, Lin; Qu, Biao; Wu, Pei; Kuang, Sheng-Yao; Jiang, Jun; Tang, Ling; Tang, Wu-Neng; Zhang, Yong-An; Zhou, Xiao-Qiu; Liu, Yang
• Is Part Of: Fish & shellfish immunology. Volume 56(2016:Sep.)
• Page Start: 111
• Page End: 122
• Publication Date: 2016-09
• Subjects: Histidine -- Gill -- Antioxidant -- Tight junction -- Cytokine -- Fish; ZO-1 zonula occludens-1 -- TNF-α tumour necrosis factor α -- IL-8 interleukin 8 -- IL-10 interleukin 10 -- TGF-β transforming growth factor β -- NF-κB nuclear factor kappa B -- IκB inhibitor protein-κB -- TOR target of rapamycin -- S6K1 ribosome protein S6 kinase 1 -- ASA anti-superoxide anion -- AHR anti-hydroxyl radical -- SOD1 copper, zinc-superoxide dismutase -- GPx glutathione peroxidase -- GSH glutathione -- Nrf2 NF-E2-related factor-2 -- Keap1 Kelch-like-ECH-associated protein 1 -- ROS reactive oxygen species -- TJ tight junction -- MDA malondialdehyde -- PC protein carbonyls -- OH hydroxyl radical -- 1O2 singlet oxygen; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.07.002
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: This study firstly explored the possible effects of dietary histidine on structural integrity and the related signaling factor gene expression in the gills of fish. Young grass carp ( Ctenopharyngodon idella ) were fed with six diets containing gradual levels of histidine for 8 weeks. The results firstly demonstrated that histidine deficiency caused increases in reactive oxygen species (ROS) contents, and severe oxidative damage (lipid peroxidation and protein oxidation) in the gills of fish, which was partially due to the decreased glutathione (GSH) content and antioxidant enzyme activities [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione- S -transferase (GST) and glutathione reductase (GR)]. Further investigations indicated that histidine deficiency caused depressions of those antioxidant enzyme activities are related to the down-regulation of corresponding antioxidant enzyme genes and the related signaling factor Nrf2 mRNA levels. Meanwhile, histidine deficiency induced DNA fragmentation via up-regulation of caspase-3, caspase-8 and caspase-9 expressions that referring to the down-regulation of TOR and S6K mRNA levels. Furthermore, His deficiency down-regulated claudin-b, claudin-c, claudin-3, claudin-12, claudin-15, occludin and ZO-1 transcription in fish gills. These effects were partially related to the up-regulation of pro-inflammatory cytokines, interleukin 1β (IL-1β), interleukin 8 (IL-8), tumor necrosis factor-α (TNF-α) and related signaling factor nuclear factor κB P65 (NF-κB P65) mRNA levels, and the down-regulation of anti-inflammatory cytokines, interleukin 10 (IL-10), transforming growth factor β1 (TGF-β1) and related signaling factor IκBα mRNA levels. Excessive histidine exhibited negative effects that were similar to histidine deficiency, whereas the optimal histidine levels reversed those negative effects. Taken together, our results showed that histidine deficiency or excess impaired the structural integrity of fish gill by disrupted fish antioxidant defenses and regulating the expression of tight junction protein, cytokines, apoptosis, antioxidant enzymes, NF-κB p65, IκBα, TOR, Nrf2, Keap1 and apoptosis-related genes in the fish gills. Highlights: His deficiency caused oxidative damage and apoptosis in the gills of fish. His deficiency depressed the antioxidant ability related to the inhibition of Nrf2 signaling in fish gills. His deficiency caused tight junction protein mRNA level changes in fish gills. His deficiency altered cytokine and related molecule mRNA levels in fish gills. Excessive His impaired the structural integrity of the gills in fish.