A hemocyte-expressed fibrinogen-related protein gene (LvFrep) from the shrimp Litopenaeus vannamei: Expression analysis after microbial infection and during larval development. (September 2016)

• Record Type: Journal Article
• Authors: Coelho, Jaqueline da Rosa; Barreto, Cairé; Silveira, Amanda da Silva; Vieira, Graziela Cleusa; Rosa, Rafael Diego; Perazzolo, Luciane Maria
• Is Part Of: Fish & shellfish immunology. Volume 56(2016:Sep.)
• Page Start: 123
• Page End: 126
• Publication Date: 2016-09
• Subjects: Pattern recognition protein/receptor -- Fibrinogen-related protein -- Crustacean immunity -- Gene expression -- Vibrio harveyi; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.06.046
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Fibrinogen-related proteins (FREPs) comprise a large family of microbial recognition proteins involved in many biological functions in both vertebrate and invertebrate animals. By taking advantage of publicly accessible databases, we have identified a FREP-like homolog in the most cultivated penaeid shrimp, Litopenaeus vannamei ( Lv Frep). The obtained sequence showed a conserved fibrinogen-related domain (FReD) and displayed significant similarities to FREP-like proteins from other invertebrates and to ficolins from crustaceans. The expression of Lv Frep appeared to be limited to circulating hemocytes. Interestingly, Lv Frep gene expression was induced in shrimp hemocytes only in response to a Vibrio infection but not to the White spot syndrome virus (WSSV). Moreover, Lv Frep transcript levels were detected early in fertilized eggs, suggesting the participation of this immune-related gene in the antimicrobial defenses during shrimp development. Highlights: The Lv Frep sequence displayed similarities to other FREP-like proteins and ficolins. Lv Frep transcripts showed to be highly expressed in circulating hemocytes. Lv Frep expression was induced in response to a Vibrio infection but not to the WSSV. Lv Frep transcripts were detected in all stages of shrimp development.