Differential immune gene expression profiles in susceptible and resistant full-sibling families of Atlantic salmon (Salmo salar) challenged with infectious pancreatic necrosis virus (IPNV). Issue 1 (November 2015)

• Record Type: Journal Article
• Authors: Reyes-López, Felipe E.; Romeo, Jose S.; Vallejos-Vidal, Eva; Reyes-Cerpa, Sebastián; Sandino, Ana M.; Tort, Lluis; Mackenzie, Simon; Imarai, Mónica
• Is Part Of: Developmental and comparative immunology. Volume 53:Issue 1(2015)
• Page Start: 210
• Page End: 221
• Publication Date: 2015-11
• Subjects: IPN-Resistance -- Full-sibling families -- Salmo salar -- Transcriptomics -- Microarrays -- Immune response; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2015.06.017
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: This study aims to identify at the expression level the immune-related genes associated with IPN-susceptible and resistant phenotypes in Atlantic salmon full-sibling families. We have analyzed thirty full-sibling families infected by immersion with IPNV and then classified as resistant or susceptible using a multivariate survival analysis based on a gamma-Cox frailty model and the Kaplan–Meier mortality curves. In four families within each group head kidneys were pooled for real-time PCR and one-color salmon-specific oligonucleotide microarray (21K) analysis at day 1 and 5 post-infection. Transcripts involved in innate response (IL-6, IFN-α), antigen presentation (HSP-70, HSP-90, MHC-I), TH 1 response (IL-12, IFN-γ, CRFB6), immunosuppression (IL-10, TGF-β1) and leukocyte activation and migration (CCL-19, CD18) showed a differential expression pattern between both phenotypes, except in IL-6. In susceptible families, except for IFN-γ, the expressions dropped to basal values at day 5 post-infection. In resistant families, unlike susceptible families, levels remained high or increased (except for IL-6) at day 5. Transcriptomic analysis showed that both families have a clear differential expression pattern, resulting in a marked down-regulation in immune related genes involved in innate response, complement system, antigen recognition and activation of immune response in IPN-resistant. Down-regulation of genes, mainly related to tissue differentiation and protein degradation metabolism, was also observed in resistant families. We have identified an immune-related gene patterns associated with susceptibility and resistance to IPNV infection of Atlantic salmon. This suggests that a limited immune response is associated with resistant fish phenotype to IPNV challenge while a highly inflammatory but short response is associated with susceptibility. Highlights: Full-sibling families of Atlantic salmon were infected by immersion with IPNV. Four IPN-susceptible and -resistant families were analyzed by qPCR and microarray. Immune-related transcripts differentiate both salmon families. A highly inflammatory but short response is associated with susceptibility.