Transcriptional dynamics of immune, growth and stress related genes in skeletal muscle of the fine flounder (Paralichthys adpersus) during different nutritional statuses. Issue 1 (November 2015)

• Record Type: Journal Article
• Authors: Valenzuela, Cristián A.; Escobar, Daniela; Perez, Lorena; Zuloaga, Rodrigo; Estrada, Juan Manuel; Mercado, Luis; Valdés, Juan Antonio; Molina, Alfredo
• Is Part Of: Developmental and comparative immunology. Volume 53:Issue 1(2015)
• Page Start: 145
• Page End: 157
• Publication Date: 2015-11
• Subjects: Fish -- Nutritional status -- Skeletal muscle -- Growth -- Stress -- Immunity; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2015.06.007
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: The effects of stress on immune activity and growth in early vertebrates have not been studied in detail. The present study used fine flounder ( Paralichthys adspersus ) skeletal muscle as a model to evaluate molecules involved in the stress response, including the glucocorticoid receptors, foxo1/3, and the target genes of these. Additionally, immune markers ( il-1β and tnfα ) and effector molecules of atrophy ( bnip3, caspase-3, and lc3 ) were assessed. These molecules were analyzed during periods of long-term fasting and refeeding. During fasting, gene expression related to the stress response and atrophy increased; whereas immune markers were down-regulated. During refeeding, atrophy- and stress-related gene expression significantly decreased. In contrast, immune markers were up-regulated. These results provide novel insight on the control of growth in the skeletal muscle of a non-mammalian species under a stressful condition, suggesting that growth, stress, and immune activity in muscle are closely related and coordinated by orchestrated transcriptional dynamics. Graphical abstract: Highlights: The fine flounder is a unique model to study adverse conditions in muscle. An immune suppression occurs during fasting period. Immune related genes are up-regulated during refeeding period. During short term refeeding, stress molecules were down-regulated. The results show a correlation between immune, growth and stress related genes.