Immunotoxicity of commercial-mixed glyphosate in broad snouted caiman (Caiman latirostris). (25th January 2016)

• Record Type: Journal Article
• Authors: Siroski, Pablo A.; Poletta, Gisela L.; Latorre, María A.; Merchant, Mark E.; Ortega, Hugo H.; Mudry, Marta D.
• Is Part Of: Chemico-biological interactions. Volume 244:(2016)
• Page Start: 64
• Page End: 70
• Publication Date: 2016-01-25
• Subjects: Broad snouted caiman -- Complement system -- White blood cell -- Glyphosate -- Immunotoxicity; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2015.11.031
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: The expansion and intensification of agriculture during the past 50 years is unprecedented, and thus environmental problems have been triggered at different scales. These transformations have caused the loss of habitat and biodiversity, and disruption of the structure and functioning of ecosystems. As a result of the expansion of the agricultural frontier in the recent past, many areas of the natural geographic distribution of the local wildlife, among them crocodilians and particularly the broad snouted caiman ( Caiman latirostris ), are being exposed to contaminants. The present study was designed to evaluate the effect of commercially-mixed glyphosate (RU) on some parameters of the immune system of C. latirostris . Two groups of caimans were exposed for two months to different concentrations of RU recommended for its application in the field, while one group was maintained as an unexposed control. The RU concentration was progressively decreased through the exposure period to simulate glyphosate degradation in water. After exposure, total and differential white blood cell (WBC), and complement system activity (CS) were determined. In addition, the animals were injected with a solution of lipopolysaccharide (LPS) from Escherichia coli to trigger an immune response and evaluate the parameters associated with it. The results showed that an effect of the herbicide on CS was observed, as animals exposed to RU showed a lower CS activity than animals from the negative control (NC) but not in total WBC. In the case of leukocyte population counts, differences were only found for heterophils and lymphocytes. Highlights: Caiman latirostris can be exposed to contaminants in all stages of life. The innate immunity can be altered for release of toxic substances to the environment. C. latirostris hatchlings were exposed to subchronic concentrations of Glyphosate. Different concentrations of pesticides negatively affect the activity of the CS. The suppression of immune function can lead to increased risk of diseases in reptiles.