Structural characterization of a branched (1 → 6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius. (1st August 2016)

• Record Type: Journal Article
• Authors: Nyman, Anna Armika T.; Aachmann, Finn L.; Rise, Frode; Ballance, Simon; Samuelsen, Anne Berit C.
• Is Part Of: Carbohydrate polymers. Volume 146(2016)
• Page Start: 197
• Page End: 207
• Publication Date: 2016-08-01
• Subjects: SEC-MALLS Size Exclusion Chromatography-Multi-Angle Laser Light Scattering -- OCc Cantharellus cibarius organic extract -- ECc Cantharellus cibarius ethanolic extract -- WCc Cantharellus cibarius water extract -- ACc Cantharellus cibarius alkali extract -- ACcIw Cantharellus cibarius alkali extract insoluble in water -- ACcSw Cantharellus cibarius alkali extract soluble in water -- ACcId Cantharellus cibarius insoluble alkali extract insoluble in dimethyl sulfoxide -- ACcSd Cantharellus cibarius insoluble alkali extract soluble in dimethyl sulfoxide; Basidiomycota -- Chanterelle -- Saccharomyces cerevisiae -- Mannan -- Glucan -- NMR; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.03.052
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: The chanterelle cell wall contains an (1 → 6)-α-mannan, (1 → 6)- and (1 → 3)-β-glucans. The α-mannan is a water soluble polysaccharide extracted in boiling water. The release of β-glucans requires treatment with hot alkali solution. Abstract: A comprehensive characterization of polysaccharides from the chanterelle was performed. Experiments included both linkage analysis by methylation and GC–MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC. A 671 kDa (1 → 6)-linked α-d -mannan with single and short (1 → 2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography. The hot 1 M NaOH extract contained two types of β-glucan; a water soluble (1 → 6)-linked β-d -glucan with single and short (1 → 3)-linked β-glucan side chains (ACcSw) and a less branched (1 → 3)-linked β-d -glucan (ACcIw/ACcId), proposed to contain short side chains in O -4. Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1 → 6)/(1 → 3)-β-d -glucan derived from Saccharomyces cerevisiae which was used as reference.