Regeneration of islet β‐cells in tree shrews and rats. Issue 2 (28th July 2018)

• Record Type: Journal Article
• Authors: Zhao, Yu‐Qiong; Liu, Ya‐Qian; Yuan, Ji‐Fang; Dai, Xin; Niu, Miao‐Miao; Sun, Xiao‐Mei; Kuang, De‐Xuan; Wang, Wen‐Guang; Tong, Pin‐Fen; Li, Na; Xiang, Lei; Jia, Yun‐Xiao; Dai, Jie‐Jie; Chen, Hua
• Is Part Of: Animal models and experimental medicine. Volume 1:Issue 2(2018)
• Page Start: 152
• Page End: 161
• Publication Date: 2018-07-28
• Subjects: β‐cell -- rat -- regeneration -- tree shrew; Laboratory animals -- Periodicals; Diseases -- Animal models -- Periodicals; Animal models in research -- Periodicals; Veterinary medicine -- Periodicals; Laboratory Animal Science; Disease Models, Animal; Animals, Laboratory; Animal Welfare; Veterinary Medicine; Animal models in research; Diseases -- Animal models; Laboratory animals; Veterinary medicine; Periodicals; Fulltext; Internet Resources; Periodicals; Periodicals; 616.0273
• DOI: 10.1002/ame2.12023
• Languages: English
• ISSNs: 2576-2095

Abstract

Abstract: Backgroud: Current understanding of injury and regeneration of islet β‐cells in diabetes is mainly based on rodent studies. The tree shrew is now generally accepted as being among the closest living relatives of primates, and has been widely used in animal experimentation. However, there are few reports on islet cell composition and regeneration of β‐cells in tree shrews. Methods: In this study, we examined the changes in islet cell composition and regeneration of β‐cells after streptozotocin (STZ) treatment in tree shrews compared with Sprague‐Dawley rats. Injury and regeneration of islet β‐cells were observed using hematoxylin and eosin (HE) staining and immunohistochemical staining for insulin, glucagon, somatostatin and PDX‐1. Results: Our data showed that in rats islet injury was most obvious on day 3 after injection, and islet morphologies were significantly restored by day 21. Regeneration of islet β‐cells was very pronounced in rats, and mainly involved regeneration of centro‐acinar cells and transformation of extra‐islet ductal cells. In tree shrews, the regeneration of islet β‐cells was not as significant. On days 3 and 7, only scattered regenerated cells were observed in the remaining islets. Further, no regeneration of centro‐acinar cells was observed. Conclusion: The results suggest that the repair mechanism of islet β‐cells in tree shrews is similar to that of humans.