Restraint‐based three‐dimensional modeling of genomes and genomic domains. Issue 20 (14th May 2015)

• Record Type: Journal Article
• Authors: Serra, François; Di Stefano, Marco; Spill, Yannick G.; Cuartero, Yasmina; Goodstadt, Michael; Baù, Davide; Marti-Renom, Marc A.
• Is Part Of: FEBS letters. Volume 589:Issue 20(2015) Part A
• Page Start: 2987
• Page End: 2995
• Publication Date: 2015-05-14
• Subjects: Genome architecture -- 3D genome reconstruction -- Chromosome Conformation Capture -- Restraint-based modeling; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2015.05.012
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Chromosomes are large polymer molecules composed of nucleotides. In some species, such as humans, this polymer can sum up to meters long and still be properly folded within the nuclear space of few microns in size. The exact mechanisms of how the meters long DNA is folded into the nucleus, as well as how the regulatory machinery can access it, is to a large extend still a mystery. However, and thanks to newly developed molecular, genomic and computational approaches based on the Chromosome Conformation Capture (3C) technology, we are now obtaining insight on how genomes are spatially organized. Here we review a new family of computational approaches that aim at using 3C‐based data to obtain spatial restraints for modeling genomes and genomic domains.