Intratesticular alpha1-adrenergic receptors mediate stress-disturbed transcription of steroidogenic stimulator NUR77 as well as steroidogenic repressors DAX1 and ARR19 in Leydig cells of adult rats. (5th September 2015)

• Record Type: Journal Article
• Authors: Stojkov-Mimic, Natasa J.; Bjelic, Maja M.; Radovic, Sava M.; Mihajlovic, Aleksandar I.; Sokanovic, Srdjan J.; Baburski, Aleksandar Z.; Janjic, Marija M.; Kostic, Tatjana S.; Andric, Silvana A.
• Is Part Of: Molecular and cellular endocrinology. Volume 412(2015)
• Page Start: 309
• Page End: 319
• Publication Date: 2015-09-05
• Subjects: Leydig cells -- Stress -- Intratesticular α1-ADRs -- Nur77 -- Arr19 -- Dax1; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2015.05.019
• Languages: English
• ISSNs: 0303-7207

Abstract

Highlights: In vivo blockade of testicular α1-ADRs reduces circulating androgen level. α1-ADR blockade abolished stress-induced partial recovery of androgen production. α1-ADRs mediate stress-reduced steroidogenic stimulator Nur77 in Leydig cells. α1-ADRs mediate stress-increased repressors Dax1 and Arr19 in Leydig cells. α1-ADRs on Leydig cell regulate transcription of steroidogenic stimulator Nur77 . Graphical Abstract: Abstract: The aim of the present study was to define the role of testicular α1-adrenergic receptors (α1-ADRs) in stress-triggered adaptation of testosterone-producing Leydig cells of adult rats. Results showed that in vivo blockade of testicular α1-ADRs prevented partial recovery of circulating androgen levels registered after 10× repeated immobilization stress (10 × IMO). Moreover, α1-ADR-blockade diminished 10 × IMO-triggered recovery of Leydig cell androgen production, and abolished mitochondrial membrane potential recovery. In the same cells, 10 × IMO-induced increase in Star transcript was abolished, Lhcgr transcript decreased, while transcription of other steroidogenic proteins was not changed. α1-ADR-blockade recovered stress-induced decrease of Nur77, one of the main steroidogenic stimulator, while significantly reduced 10 × IMO-increased in the transcription of the main steroidogenic repressors, Arr19 and Dax1 . In vitro experiments revealed an adrenaline-induced α1-ADR-mediated decrease in Nur77 transcription in Leydig cells. Adrenaline-induced increase of repressor Dax1 also involves ADRs in Leydig cells. Accordingly, α1-ADRs participate in some of the stress-triggered effects on the steroidogenic machinery of Leydig cells.