Real-time quantitative PCR assay with Taqman® probe for rapid detection of MCR-1 plasmid-mediated colistin resistance. (September 2016)

• Record Type: Journal Article
• Authors: Chabou, S.; Leangapichart, T.; Okdah, L.; Le Page, S.; Hadjadj, L.; Rolain, J.-M.
• Is Part Of: New microbes and new infections. Volume 13(2016)
• Page Start: 71
• Page End: 74
• Publication Date: 2016-09
• Subjects: Antibiotic resistance surveillance -- colistin resistance -- mcr-1 gene -- PCR detection, Taqman probe; Medical microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; Microbiology -- Periodicals; Communicable Diseases -- Periodicals; Infection -- Periodicals; 616.9041
• DOI: 10.1016/j.nmni.2016.06.017
• Languages: English
• ISSNs: 2052-2975

Abstract

Abstract: Here we report the development of two rapid real-time quantitative PCR assays with TaqMan ® probes to detect the MCR-1 plasmid-mediated colistin resistance gene from bacterial isolates and faecal samples from chickens. Specificity and sensitivity of the assay were 100% on bacterial isolates including 18 colistin-resistant isolates carrying the mcr-1 gene (six Klebsiella pneumoniae and 12 Escherichia coli ) with a calibration curve that was linear from 10 1 to 10 8 DNA copies. Five out of 833 faecal samples from chickens from Algeria were positive, from which three E. coli strains were isolated and confirmed to harbour the mcr-1 gene by standard PCR and sequencing.