Gender- and region-dependent changes of redox biomarkers in the brain of successfully aging LOU/C rats. (July 2015)

• Record Type: Journal Article
• Authors: Moyse, Emmanuel; Arseneault, Madeleine; Gaudreau, Pierrette; Ferland, Guylaine; Ramassamy, Charles
• Is Part Of: Mechanisms of ageing and development. Volume 149(2015)
• Page Start: 19
• Page End: 30
• Publication Date: 2015-07
• Subjects: Glutathione -- Superoxide dismutase-1 -- Thioredoxine-1 -- Glutaredoxine-1 -- Protein carbonyl -- Clusterin; Aging -- Periodicals; Developmental biology -- Periodicals; Aging -- Periodicals; Developmental Biology -- Periodicals; Vieillissement -- Périodiques; Biologie du développement -- Périodiques; Aging; Developmental biology; Periodicals; 612.67
• DOI: 10.1016/j.mad.2015.04.002
• Languages: English
• ISSNs: 0047-6374

Abstract

Highlights: The oxidoreductive status of 4 brain regions is characterized across "successful healthy ageing" of the LOU rat strain. Brain levels of GSH, γ-GCS, total thiols remain constant with age in LOU rat, except for GSH and γ-GCS decreases in females. Clusterin, TRX-1, GLRX-1, SOD-1 mRNA levels are maintained or increased in the hippocampus with age. Age-dependency of the markers differ between sexes and are compared with published data from other rodent strains. Our results support the oxidative stress theory of aging. Abstract: The LOU/C (LOU) rat is an obesity resistant strain with higher longevity and healthspan than common rats. The management of oxidative stress being important to successful aging, we characterized this process in the aging LOU rat. Male/female LOU rats were euthanized at 4, 20, and 29 months. Macrodissected hippocampus, striatum, parietal cortex, cerebellum were assayed for tissue concentrations of glutathione (GSH), gamma-glutamyl-cysteine-synthetase (γ-GCS), total thiols, protein carbonyls, mRNAs of clusterin and the known protective enzymes thioredoxine-1 (TRX-1), glutaredoxine-1 (GLRX-1), superoxide dismutase-1 (SOD-1). Brain levels of GSH, γ-GCS, total thiols remained constant with age, except for GSH and γ-GCS which decreases in females. Clusterin, TRX-1, GLRX-1, SOD-1 mRNA levels were maintained or increased in the hippocampus with age. Age-dependency of the markers differed between sexes, with SOD-1 and TRX-1 decreases out of hippocampus in females. Since antioxidants were reported to decrease with age in the brain of Wistar rats, maintenance of GSH levels and of protective enzymes mRNA levels in the LOU rat brain could contribute to the preservation of cognitive functions in old age. Altogether, the successful aging of LOU rats may, at least in part, involve the conservation of functional antioxidant mechanisms in the brain, supporting the oxidative stress theory of aging.