Carbendazim resistance and dimethachlone sensitivity of field isolates of Sclerotinia sclerotiorum from oilseed rape in Henan Province, China. Issue 10 (13th July 2018)

• Record Type: Journal Article
• Authors: Liu, Shengming; Zhang, Yong; Jiang, Jia; Che, Zhiping; Tian, Yuee; Chen, Genqiang
• Is Part Of: Journal of phytopathology. Volume 166:Issue 10(2018)
• Page Start: 701
• Page End: 708
• Publication Date: 2018-07-13
• Subjects: baseline sensitivity -- fungicide -- Sclerotinia sclerotiorum -- Sclerotinia stem rot; Plant diseases -- Periodicals; 632
• DOI: 10.1111/jph.12751
• Languages: English
• ISSNs: 0931-1785

Abstract

Abstract: Sclerotinia stem rot caused by Sclerotinia sclerotiorum is one of the most important diseases in oilseed rape‐growing areas of China. To determine the frequency of resistance of field isolates of S. sclerotiorum to carbendazim and dimethachlone, a total of 556 isolates from 10 different regions of Henan Province were obtained between 2015 and 2016. The frequency of isolates with a high‐resistance phenotype and a moderate‐resistance phenotype to carbendazim was 69.2% and 10.8%, respectively. However, S. sclerotiorum isolates resistant to dimethachlone were not detected. The baseline sensitivity of S. sclerotiorum to dimethachlone was distributed as a unimodal curve with a mean EC50 value of 0.39 ± 0.09 μg ml −1 for the inhibition of mycelial growth. Four dimethachlone‐resistant mutants were obtained from 20 wild‐type isolates induced by exposure to increasing concentrations of the fungicide in vitro. The mutants showed high levels of resistance to dimethachlone, with resistance factors that ranged from 179 to 323. Positive cross‐resistance occurred between dimethachlone and procymidone, iprodione, and fludioxonil; however, no cross‐resistance was observed for carbendazim and boscalid. The fitness of the dimethachlone‐resistant mutants was significantly lower than that of the wild‐type isolates, as measured by mycelial growth, hyphal dry weight, sclerotium number and dry weight, and pathogenicity. Additionally, based on osmotic tests, the inhibition of mycelial growth caused by NaCl applied at different concentrations was significantly higher for the dimethachlone‐resistant mutants than for their wild‐type parents.