Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. (29th June 2018)

• Record Type: Journal Article
• Authors: Hamelian, Mona; Zangeneh, Mohammad Mahdi; Amisama, Abbas; Varmira, Kambiz; Veisi, Hojat
• Is Part Of: Applied organometallic chemistry. Volume 32:Number 9(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-06-29
• Subjects: antibacterial potential -- cytotoxicity properties -- green chemistry -- silver nanoparticles -- Thymus Kotschyanus; Organometallic chemistry -- Periodicals; Organometallic compounds -- Periodicals; 547.05
• DOI: 10.1002/aoc.4458
• Languages: English
• ISSNs: 0268-2605

Abstract

Abstract : Present study used ecofriendly, cost efficient and easy method for synthesis of silver nanoparticles (Ag NPs) at the room temperature by Thymus Kotschyanus extract as reducing and capping agent. Various analytical technique including UV–Vis absorption spectroscopy determined presence of Ag NPs in the solution, the functional groups of Thymus Kotschyanus extract in the reduction and capping process of Ag NPs are approved by FT‐IR, crystallinity with the fcc plane approved from the X‐ray diffraction (XRD) pattern, energy dispersive spectroscopy (EDS) determined existence of elements in the sample, surface morphology, diverse shapes and size of present Ag NPs were showed by using scanning electron microscopy (SEM), atomic force microscopy (AFM) and high resolution transmission electron microscopy (HRTEM). Beginning and end destroy temperature of present silver nanoparticles were determined by thermal gravimetric spectroscopy (TGA). In addition, antibacterial, antioxidant and cytotoxicity properties of Ag NPs were studied. Agar disk and agar well diffusion are the methods to determined antibacterial properties of synthesized Ag NPs. Also MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were recognized by macro broth dilution assay. DPPH free radical scavenging assay was used for antioxidant property and compare to butylated hydroxytoluene (BHT) as standard antioxidant that showed high antioxidant activity more than BHT. Synthesized Ag NPs have great cell viability in a dose depended manner and demonstrate that this method for synthesis silver nanoparticles provided nontoxic. The average diameter of synthesized Ag NPs was about 50–60 nm. Abstract : Ecofriendly, cost efficient and easy method for synthesis silver nanoparticles. Thymus Kotschyanus extract as reducing and capping agent. The average diameter of synthesized Ag NPs was about 50–60 nm. Its show good antibacterial, antioxidant and cytotoxicity properties.