Bacterial community shifts associated with high abundance of Rhizobium spp. in potato roots under macronutrient-deficient conditions. (January 2015)

• Record Type: Journal Article
• Authors: Unno, Yusuke; Shinano, Takuro; Minamisawa, Kiwamu; Ikeda, Seishi
• Is Part Of: Soil biology and biochemistry. Volume 80(2015)
• Page Start: 232
• Page End: 236
• Publication Date: 2015-01
• Subjects: Bacterial community -- Fertilization -- Potato -- Rhizobium -- Root-associated bacteria; Soil biochemistry -- Periodicals; Soil biology -- Periodicals; Sols -- Biochimie -- Périodiques; Sols -- Biologie -- Périodiques; Sols -- Microbiologie -- Périodiques; Bodembiologie; Biochemie; 631.46
• DOI: 10.1016/j.soilbio.2014.10.002
• Languages: English
• ISSNs: 0038-0717

Abstract

Abstract: We evaluated the impacts of a macronutrient deficiency on the community structure of root-associated bacteria in potato ( Solanum tuberosum ) cultivation. Potato plants were cultivated in a long-term experimental field under a nitrogen, phosphorus, or potassium deficiency, and roots were sampled at the early flowering stage. Amplicon libraries of 16S rRNA gene were constructed for root-associated bacteria, and bacterial diversity was analyzed by means of pyrosequencing. Statistical analyses showed significantly lower species evenness in the nutrient-deficient plots compared with the value in a plot with standard fertilization. Phylogenetic analyses revealed that the abundance of Rhizobium spp. increased dramatically in all plots under a macronutrient deficiency. Highlights: Diversity of potato root-associated bacteria in nutrient deficiency was examined. Root-associated bacteria in nutrient deficiency showed low evenness. Abundance of Rhizobium sp. was dramatically increased in nutrient deficiency.