Microsatellites characteristics analysis and SSR marker development for Paphiopedilum hirsutissimum based on transcriptome sequencing. Issue 4 (5th February 2018)

• Record Type: Journal Article
• Authors: Chen, Lin; Dong, Hui; Wang, Jianwen; Li, Longna; Xu, Meng
• Is Part Of: Plant genetic resources. Volume 16:Issue 4(2018)
• Page Start: 394
• Page End: 396
• Publication Date: 2018-02-05
• Subjects: genetic diversity, -- Paphiopedilum hirsutissimum, -- SSR, -- transcriptome sequencing; Plant genetics -- Periodicals; Plant breeding -- Periodicals; 632.523
• DOI: 10.1017/S1479262117000375
• Languages: English
• ISSNs: 1479-2621

Abstract

Abstract: Paphiopedilum hirsutissimum (Lindl. ex Hook. f.) Stein (Orchidaceae) is a valuable but endangered ornamental plant. In the present study, the microsatellites characteristics were analysed and 14 polymorphic simple sequence repeat (SSR) markers were developed based on the de novo transcriptome sequencing for P. hirsutissimum using Illumina pair-end sequencing technology. A total of 7406 SSRs were detected in 56, 688 unigenes, with the occurrence frequency of 13.06% and an average density of one SSR per 5.42 kb; and 150 primer pairs randomly selected from 2090 pairs except mononucleotide repeats were synthesized for verification. These loci were validated in 32 individuals from the concentrated distribution centre, and were successfully amplified using 14 primer sets showing a high level of polymorphism. These SSR markers will be useful in future studies of genetic diversity, the population structure of P. hirsutissimum and make effective conservation strategy.