Renewable Time‐Responsive DNA Circuits. Issue 33 (18th July 2018)

• Record Type: Journal Article
• Authors: Garg, Sudhanshu; Shah, Shalin; Bui, Hieu; Song, Tianqi; Mokhtar, Reem; Reif, John
• Is Part Of: Small. Volume 14:Issue 33(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-07-18
• Subjects: DNA computing -- enzyme‐free circuits -- logic gates -- renewable circuits; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201801470
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: DNA devices have been shown to be capable of evaluating Boolean logic. Several robust designs for DNA circuits have been demonstrated. Some prior DNA‐based circuits are use‐once circuits since the gate motifs of the DNA circuits get permanently destroyed as a side effect of the computation, and hence cannot respond correctly to subsequent changes in inputs. Other DNA‐based circuits use a large reservoir of buffered gates to replace the working gates of the circuit and can be used to drive a finite number of computation cycles. In many applications of DNA circuits, the inputs are inherently asynchronous, and this necessitates that the DNA circuits be asynchronous: the output must always be correct regardless of differences in the arrival time of inputs. This paper demonstrates: 1) renewable DNA circuits, which can be manually reverted to their original state by addition of DNA strands, and 2) time‐responsive DNA circuits, where if the inputs change over time, the DNA circuit can recompute the output correctly based on the new inputs, that are manually added after the system has been reset. The properties of renewable, asynchronous, and time‐responsiveness appear to be central to molecular‐scale systems; for example, self‐regulation in cellular organisms. Abstract : A renewable, time‐responsive DNA circuit is designed and implemented and has been shown to work for at least two cycles. The circuit output can change if the inputs are changed and the circuit can be reset to the original state based on manual addition of pullback strands. This introduces an alternate mechanism in DNA circuit design to be able to reuse DNA gates and perform Boolean logic computation.