PSII photochemistry, oxidative damage and anti-oxidative enzymes in arsenate-stressed Oryza sativa L. seedlings. Issue 1 (2nd January 2017)

• Record Type: Journal Article
• Authors: Mishra, Rohit Kumar; Kumar, Jitendra; Srivastava, Prabhat Kumar; Bashri, Gausiya; Prasad, Sheo Mohan
• Is Part Of: Chemistry and ecology. Volume 33:Issue 1(2017)
• Page Start: 34
• Page End: 50
• Publication Date: 2017-01-02
• Subjects: Arsenic -- chlorophyll a fluorescence -- oxidative stress -- rice -- SOD isoenzyme; Pollution -- Environmental aspects -- Periodicals; Ecology -- Periodicals; 577.2
• DOI: 10.1080/02757540.2016.1265516
• Languages: English
• ISSNs: 0275-7540

Abstract

ABSTRACT: The aim of the present study was to quantify the arsenic-induced damage in rice seedlings in terms of certain physiological and biochemical parameters. For this, hydroponically grown Oryza sativa L. seedlings were exposed to certain doses of As V as Na2 HAsO4 (10, 25, 50 and 100 µM). All the tested doses manifested in a significant loss in the pigments content and photosynthesis, which culminated in retarded growth of the seedlings. The As V -mediated decline in PSII photochemistry could be correlated with the decline in ϕP0, ψ0, ϕE0 and PIABS . In vitro and in vivo detection of and H2 O2 and the consequent rise in malondialdehyde equivalents after As V treatments stimulated the anti-oxidant enzymes, that is, superoxide dismutase (SOD) (except at 50 and 100 µM As V ), peroxidase and catalase, activity which was further confirmed by the native polyacrylamide gel electrophoresis (PAGE) that also revealed the least expression of SOD isoforms against 50 and 100 µM As V treatment. The present investigation is a categorical demonstration of the inhibitory behaviour of As V in O. sativa L. seedlings. At lower doses of arsenate, reactive oxygen species and anti-oxidants cooperatively acted in such a way that the impacts of the stress were far lesser on various growth and physiological parameters.