Effect of the accuracy of pH control on hydrogen fermentation. (March 2015)

• Record Type: Journal Article
• Authors: Moon, Chungman; Jang, Sujin; Yun, Yeo-Myeong; Lee, Mo-Kwon; Kim, Dong-Hoon; Kang, Won-Seok; Kwak, Seung-Shin; Kim, Mi-Sun
• Is Part Of: Bioresource technology. Volume 179(2015)
• Page Start: 595
• Page End: 601
• Publication Date: 2015-03
• Subjects: Hydrogen fermentation -- pH deviation -- Lactate -- Butyrate -- Next generation sequencing; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2014.10.128
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: A significant effect of pH control accuracy on H2 fermentation performance. High H2 yield and substrate utilization at deviation of ±0.1 and ±0.3. Low H2 production with lowered substrate utilization at deviation >±0.5. Lactic acid bacteria became dominant with increase of deviation value. Abstract: pH, known as the most important parameter in H2 fermentation, cannot be precisely controlled in a scaled-up fermenter as in a lab fermenter. In the preset work, to assess the effect of pH control accuracy on H2 fermentation, the pH was controlled at 6.0 ± 0.1, 6.0 ± 0.3, 6.0 ± 0.5, 6.0 ± 0.7, and 6.0 ± 0.9 during batch fermentation of food waste. Up to deviation of ±0.3, a high H2 yield of 1.67–1.73 mol H2 /mol hexoseadded was attained with producing butyrate as a major metabolite (>70% of total organic acids produced). A huge drop of H2 production, however, was observed at deviation >±0.5 with lowered substrate utilization and increased production of lactate. Next generation sequencing results showed that Clostridium was found to be the dominant genus (76.4% of total number of sequences) at deviation of ±0.1, whereas the dominant genus was changed to lactic acid bacteria such as Streptococcus and Lactobacillus with increase of deviation value.