Forensic and population genetic analysis of Xinjiang Uyghur population on 21 short tandem repeat loci of 6-dye GlobalFiler™ PCR Amplification kit. (May 2016)

• Record Type: Journal Article
• Authors: Zhang, Honghua; Xia, Mingying; Qi, Lijie; Dong, Lei; Song, Shuang; Ma, Teng; Yang, Shuping; Jin, Li; Li, Liming; Li, Shilin
• Is Part Of: Forensic science international. Volume 22(2016:May)
• Page Start: 22
• Page End: 24
• Publication Date: 2016-05
• Subjects: Short tandem repeat -- GlobalFiler -- Uyghur -- Xinjiang; Forensic genetics -- Periodicals; Génétique légale -- Périodiques; Forensic genetics; Electronic journals; Periodicals; 614.1
• DOI: 10.1016/j.fsigen.2016.01.005
• Languages: English
• ISSNs: 1872-4973

Abstract

Highlights: We report allele frequencies and forensic parameters on 21 autosomal STR loci of the 6-dye GlobalFiler™ PCR Amplification kit on the Chinese Uyghur population in Xinjiang. No significant deviations from Hardy–Weinberg equilibrium and linkage disequilibrium were detected within and between the GlobalFiler STR loci. We find that SE33 showed the greatest power of discrimination in Uyghur population, whereas TPOX showed the lowest. No significant difference was observed between Uyghur and other Chinese populations at nearly all tested STRs, as well as Central-South Asian and East Asian. The phylogenetic analysis showed that Uyghur is genetically close to Chinese populations, as well as East Asian and Central-South Asian. Abstract: Estimating the allele frequencies and forensic statistical parameters of commonly used short tandem repeat (STR) loci of the Uyghur population, which is the fifth largest group in China, provides a more precise reference database for forensic investigation. The 6-dye GlobalFiler™ Express PCR Amplification kit incorporates 21 autosomal STRs, which have been proven that could provide reliable DNA typing results and enhance the power of discrimination. Here we analyzed the GlobalFiler STR loci on 1962 unrelated individuals from Chinese Uyghur population of Xinjiang, China. No significant deviations from Hardy–Weinberg equilibrium and linkage disequilibrium were detected within and between the GlobalFiler STR loci. SE33 showed the greatest power of discrimination in Uyghur population, whereas TPOX showed the lowest. The combined power of discrimination was 99.999999999999999999999998746%. No significant difference was observed between Uyghur and the other two Uyghur populations at all tested STRs, as well as Dai and Mongolian. Significant differences were only observed between Uyghur and other Chinese populations at TH01, as well as Central-South Asian at D13S317, East Asian at TH01 and VWA. The phylogenetic analysis showed that Uyghur is genetically close to Chinese populations, as well as East Asian and Central-South Asian.