Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium. (January 2017)

• Record Type: Journal Article
• Authors: Song, Mengke; Yang, Ying; Jiang, Longfei; Hong, Qing; Zhang, Dayi; Shen, Zhenguo; Yin, Hua; Luo, Chunling
• Is Part Of: Environmental pollution. Volume 220:Part B(2017)
• Page Start: 1059
• Page End: 1067
• Publication Date: 2017-01
• Subjects: Bioremediation -- Phenanthrene (PHE) -- Copper -- Sphingobium -- PAH-RHDα gene -- C23O gene; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2016.11.037
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: A copper-tolerant phenanthrene (PHE)-degrading bacterium, strain Sphingobium sp. PHE-1, was newly isolated from the activated sludge in a wastewater treatment plant. Two key genes, ahdA1b-1 encoding polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHDɑ) and xyLE encoding catechol-2, 3-dioxygenase (C23O), involved in the PHE metabolism by strain PHE-1 were identified. The PAH-RHD gene cluster showed 96% identity with the same cluster of Sphingomonas sp. P2. Our results indicated the induced transcription of xylE and ahdA1b-1 genes by PHE, simultaneously promoted by Cu(II). For the first time, high concentration of Cu(II) is found to encourage the expression of PAH-RHDɑ and C23O genes during PHE degradation. Applying Sphingomonas PHE-1 in PHE-contaminated soils for bioaugmentation, the abundance of xylE gene was increased by the planting of ryegrass and the presence of Cu(II), which, in turn, benefited ryegrass growth. The best performance of PHE degradation and the highest abundance of xylE genes occurred in PHE-copper co-contaminated soils planted with ryegrass. Graphical abstract: Highlights: A newly isolated bacterium possesses high PHE biodegradability and Cu-tolerance. High level Cu was reported to promote PAH-RHDɑ and C23O genes expression. Structure of PAH-RHD gene cluster has high similarity to other Sphingobium strains. Ryegrass and Cu enhanced PHE degradation and abundance of Sphingobium PHE-1. Abstract : The PHE biodegradability and expression of PHE degradation genes in a newly isolated bacterium strain were enhanced by high level copper.