Assessment of chlorpyrifos toxic effects in zebrafish (Danio rerio) metabolism. (January 2017)

• Record Type: Journal Article
• Authors: Gómez-Canela, Cristian; Prats, Eva; Piña, Benjamí; Tauler, Romà
• Is Part Of: Environmental pollution. Volume 220:Part B(2017)
• Page Start: 1231
• Page End: 1243
• Publication Date: 2017-01
• Subjects: Zebrafish -- Environmental pollution -- LC-HRMS -- Metabolomics -- ROI-MCR-ALS; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2016.11.010
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: In this work the effect of chlorpyrifos exposure on metabolic profiles of zebrafish muscle was evaluated by liquid chromatography coupled to high resolution mass spectrometry. Different chemometric tools based on the selection of Regions of Interest and on Multivariate Curve-Resolution-Alternating Least Squares are proposed for the analysis of the complex data sets generated in the different exposure experiments. Analysis of Variance Simultaneous Component Analysis of changes on metabolite peak profile areas showed significant chlorpyrifos concentration and exposure time-dependent changes, clearly differentiating between exposed and non-exposed samples and between short (2 h) and long exposure times (6 h or 24 h). The changes observed in the concentrations of 50 muscle metabolites are indicative of induction of oxidative stress, of a general disruption of neurotransmitter metabolism, and of muscle exhaustion. These three effects are intimately related to the toxicity of chlorpyrifos. Graphical abstract: Highlights: Metabolites of zebrafish were determined following LC-HRMS and different chemometric strategies. ASCA revealed that chlorpyrifos (CPF) dose and exposure times produced significant changes in metabolite concentrations. Samples exposed at 5 μM and 2 μM CPF showed dose-dependent effects regarding to control samples. Observed changes in metabolome of zebrafish were consistent with the induction of oxidative stress in CPF exposed samples. Abstract : The combination of LC-HRMS and chemometric tools allowed the postulation of affected metabolic pathways by the action of chlorpyrifos on the zebrafish muscle.