Biodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB-8T. Issue 1 (15th April 2016)

• Record Type: Journal Article
• Authors: Gentile, G.; Bonsignore, M.; Santisi, S.; Catalfamo, M.; Giuliano, L.; Genovese, L.; Yakimov, M.M.; Denaro, R.; Genovese, M.; Cappello, S.
• Is Part Of: Marine pollution bulletin. Volume 105:Issue 1(2016:Apr.)
• Page Start: 125
• Page End: 130
• Publication Date: 2016-04-15
• Subjects: Bioremediation -- Hydrocarbonoclastic bacteria -- Oil spill -- Oleispira antarctica -- Psychrophilic bacteria; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2016.02.041
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: The present study is focused on assessing the growth and hydrocarbon-degrading capability of the psychrophilic strain Oleispira antarctica RB-8 T . This study considered six hydrocarbon mixtures that were tested for 22 days at two different cultivation temperatures (4 and 15 °C). During the incubation period, six sub-aliquots of each culture at different times were processed for total bacterial abundance and GC–FID (gas chromatography–flame ionization detection) hydrocarbon analysis. Results from DNA extraction and DAPI (4′, 6-diamidino-2-phenylindole) staining showed a linear increase during the first 18 days of the experiment in almost all the substrates used; both techniques showed a good match, but the difference in values obtained was approximately one order of magnitude. GC–FID results revealed a substantial hydrocarbon degradation rate in almost all hydrocarbon sources and in particular at 15 °C rather than 4 °C (for commercial oil engine, oily waste, fuel jet, and crude oil). A more efficient degradation was observed in cultures grown with diesel and bilge water at 4 °C. Highlights: This study investigated biodegradation in cold marine environments. Treatment of naval transportation wastes is essential. Oleispira sp. is a potential candidate that plays a key role in the cleanup treatments of such wastes.