High-speed Raman imaging of cellular processes. (August 2016)

• Record Type: Journal Article
• Authors: Ando, Jun; Palonpon, Almar F; Sodeoka, Mikiko; Fujita, Katsumasa
• Is Part Of: Current opinion in chemical biology. Volume 33(2016)
• Page Start: 16
• Page End: 24
• Publication Date: 2016-08
• Subjects: Bioorganic chemistry -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Clinical biochemistry -- Periodicals; Biochemistry -- Periodicals; Chimie bio-organique -- Périodiques; Biologie -- Périodiques; 572.05
• DOI: 10.1016/j.cbpa.2016.04.005
• Languages: English
• ISSNs: 1367-5931

Abstract

Highlights: Raman microscopy with parallel spectral acquisition provides about two orders of magnitude improvement in imaging speed. High-speed Raman imaging captured various cellular processes such as cell differentiation. Raman tag allows the specific observation of small molecules in live cells. Raman tag has been applied to an organelle marker, a lipid marker, and an intracellular sensor. Abstract : Raman scattering microscopy provides information about the distribution and chemical state of molecules in live cells without any labeling or modification. In recent years, the imaging speed of Raman microscopy has improved greatly owing to the development of instruments that can perform parallel acquisition of Raman spectra from multiple points. In this article, we review recent advances in high-speed hyperspectral Raman imaging and its application to observe various biological processes such as cell mitosis, apoptosis and cell differentiation. Furthermore, we discuss the recent progress in Raman tags for the specific observation of bioactive small molecules in complex biological systems, including the development of organelle-specific probes, imaging of lipid rafts in an artificial monolayer membrane and application as a structure-sensitive tag.