Simultaneous production of triacylglycerol and high-value carotenoids by the astaxanthin-producing oleaginous green microalga Chlorella zofingiensis. (August 2016)

• Record Type: Journal Article
• Authors: Liu, Jin; Mao, Xuemei; Zhou, Wenguang; Guarnieri, Michael T.
• Is Part Of: Bioresource technology. Volume 214(2016)
• Page Start: 319
• Page End: 327
• Publication Date: 2016-08
• Subjects: Astaxanthin -- Biofuels -- Chlorella zofingiensis -- Integrated production -- Stress conditions -- Triacylglycerol; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.04.112
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Stress conditions induced TAG and astaxanthin production in C. zofingiensis. A strong correlation between TAG and astaxanthin accumulation was observed. Simultaneous TAG and astaxanthin production in semicontinuous modes was optimized. High TAG and astaxanthin productivities (297 and 3.3 mg L −1 day −1 ) were obtained. Inhibiting de novo fatty acid biosynthesis enhanced astaxanthin accumulation. Abstract: The production of lipids and astaxanthin, a high-value carotenoid, by Chlorella zofingiensis was investigated under different culture conditions. Comparative analysis revealed a good correlation between triacylglycerol (TAG) and astaxanthin accumulation in C. zofingiensis . Stress conditions promoted cell size and weight and induced the accumulation of neutral lipids, especially TAG and astaxanthin, with a concomitant decrease in membrane lipids. The highest contents of TAG and astaxanthin achieved were 387 and 4.89 mg g −1 dry weight, respectively. A semi-continuous culture strategy was developed to optimize the TAG and astaxanthin productivities, which reached 297 and 3.3 mg L −1 day −1, respectively. Additionally, astaxanthin accumulation was enhanced by inhibiting de novo fatty acid biosynthesis. In summary, our study represents a pioneering work of utilizing Chlorella for the integrated production of lipids and high-value products and C. zofingiensis has great potential to be a promising production strain and serve as an emerging oleaginous model alga.