Characterisation and diversity of Indian isolates of Ralstonia solanacearum causing bacterial wilt of Capsicum annuum L. (3rd April 2018)

• Record Type: Journal Article
• Authors: Singh, Dinesh; Chaudhary, Garima; Yadav, D. K.
• Is Part Of: Archives of phytopathology and plant protection. Volume 51:Number 5/6(2018)
• Page Start: 267
• Page End: 276
• Publication Date: 2018-04-03
• Subjects: Bacterial wilt -- biovar -- Rep-PCR -- virulence genes -- phylotyping -- race; Plants, Protection of -- Periodicals; Plant diseases -- Periodicals; 632
• DOI: 10.1080/03235408.2018.1467620
• Languages: English
• ISSNs: 0323-5408

Abstract

Abstract: Genetic diversity of 13 isolates of Ralstonia solanacearum causing bacterial wilt in hot pepper and bell pepper ( Capsicum annuum L.) from 6 states of India was assessed. All isolates of R. solanacearum belonged to biovar 3, race 1 and phylotype I. These isolates consisted of 4 distinct DNA types at 75% similarity coefficient using ERIC, BOX and REP-PCRs techniques. Multilocus sequence analysis of hrp B, fliC and egl genes of 6 isolates of R. solanacearum along with 2 out group bacteria was done and they showed high level of variability within these three regions of the genome involving in pathogenicity.