An improved pH-responsive carrier based on EDTA-Ca-alginate for oral delivery of Lactobacillus rhamnosus ATCC 53103. (2nd January 2017)

• Record Type: Journal Article
• Authors: Zheng, Huizhen; Gao, Meng; Ren, Ying; Lou, Ruyun; Xie, Hongguo; Yu, Weiting; Liu, Xiudong; Ma, Xiaojun
• Is Part Of: Carbohydrate polymers. Volume 155(2016)
• Page Start: 329
• Page End: 335
• Publication Date: 2017-01-02
• Subjects: Hydrogel -- EDTA-Ca-Alg -- pH-Responsive carrier -- Lactobacillus rhamnosus ATCC 53103 -- Controlled release; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.08.096
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A pH-responsive gel based on EDTA-Ca-Alg was achieved by in-situ release of Ca 2+ . The polysaccharide gel exhibited pH-dependent mechanical behaviors. pH-Responsive gel microspheres were formed for oral delivery of probiotics. Abstract: A pH-responsive carrier based on an ethylenediaminetetraacetic-calcium-alginate (EDTA-Ca-Alg) system was developed by controlling the release of Ca 2+ . The system remained in the solution state at neutral pH since EDTA completely chelated the Ca 2+ . In contrast, a hydrogel immediately formed when the pH was below 4.0, which triggered the in situ release of Ca 2+ from the EDTA-Ca compound and led to alginate-Ca binding. Taking advantage of the pH sensitivity, we prepared hydrogel microspheres with uniform size to entrap Lactobacillus rhamnosus ATCC 53103 through emulsification. In an acidic environment, the hydrogel structure remained compact with negligible pores to protect L. rhamnosus ATCC 53103. However, in a neutral intestinal environment, the hydrogel structure gradually disassembled because of the Ca 2+ release from the hydrogel, which caused cell release. Therefore, a pH-responsive carrier was developed for the protection and the controlled release of cells in gastrointestinal tract, thus providing potential for oral delivery of probiotics.