Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium. (August 2016)

• Record Type: Journal Article
• Authors: Muñiz-Márquez, Diana B.; Contreras, Juan C.; Rodríguez, Raúl; Mussatto, Solange I.; Teixeira, José A.; Aguilar, Cristóbal N.
• Is Part Of: Bioresource technology. Volume 213(2016)
• Page Start: 276
• Page End: 282
• Publication Date: 2016-08
• Subjects: Fructosyltransferase -- Fructooligosaccharides -- Aguamiel -- Solid State Fermentation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.03.022
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Aguamiel is a potential source for production of fructosyltransferases enzymes. FOS production in aguamiel as substrate is a viable alternative. Fructosyltransferase and FOS production by Solid State Fermentation is a viable and economic alternative. Abstract: The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347 U/L) was obtained at 32 °C, using packing density of 0.7 g/cm 3 . Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.