Novel Raman-tagged sphingomyelin that closely mimics original raft-forming behavior. Issue 13 (1st July 2015)

• Record Type: Journal Article
• Authors: Cui, Jin; Matsuoka, Shigeru; Kinoshita, Masanao; Matsumori, Nobuaki; Sato, Fuminori; Murata, Michio; Ando, Jun; Yamakoshi, Hiroyuki; Dodo, Kosuke; Sodeoka, Mikiko
• Is Part Of: Bioorganic & medicinal chemistry. Volume 23:Issue 13(2015)
• Page Start: 2989
• Page End: 2994
• Publication Date: 2015-07-01
• Subjects: Sphingomyelin -- Lipid rafts -- Raman imaging -- Solid-state 2H NMR -- Phase distribution; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; Chemistry, Clinical -- Periodicals; Chemistry, Organic -- Periodicals; Chimie bio-organique -- Périodiques; Chimie pharmaceutique -- Périodiques; 615.19
• DOI: 10.1016/j.bmc.2015.05.014
• Languages: English
• ISSNs: 0968-0896

Abstract

Graphical abstract: Abstract: Three Raman probes of sphingomyelin (SM) were synthesized and evaluated for their applicability to imaging experiments. One probe containing a hydroxymethyl-1, 3-butadiyne moiety in the polar head group showed strong scattering. The solid-state 2 H NMR spectra of this probe in oriented bilayer membrane revealed excellent compatibility with natural SM in phase behavior since the probe undergoes phase separation to form raft-like liquid ordered (Lo ) domains in the raft-mimicking mixed bilayers.