In vivo triglyceride synthesis in subcutaneous adipose tissue of humans correlates with plasma HDL parameters. (August 2016)

• Record Type: Journal Article
• Authors: Tuvdendorj, Demidmaa; Munoz, Alejandro O.; Ruiz-Barros, Viviana; Schwarz, Jean-Marc; Montalto, Giuseppe; Chandalia, Manisha; Sowers, Lawrence C.; Rizzo, Manfredi; Murphy, Elizabeth J.; Abate, Nicola
• Is Part Of: Atherosclerosis. Volume 251(2016)
• Page Start: 147
• Page End: 152
• Publication Date: 2016-08
• Subjects: Triglycerides -- Adipocyte -- HDL-C metabolism -- Stable isotope tracer kinetics -- Atheroprotective -- Gender-related -- Mass spectrometry; fTG fractional synthesis of Triglycerides -- L-HDL large HDL -- I-HDL intermediate HDL -- S-HDL small HDL -- EM1 excess mass isotopomer abundance for M1-glycerol -- A∞1 asymptotic mass isotopomer M1 abundance of a fully labeled glycerol; Arteriosclerosis -- Periodicals; Electronic journals; 616.136
• DOI: 10.1016/j.atherosclerosis.2016.06.024
• Languages: English
• ISSNs: 0021-9150

Abstract

Abstract: Backgrounds and aims: Low concentrations of plasma HDL-C are associated with the development of atherosclerotic cardiovascular diseases and type 2 diabetes. Here we aimed to explore the relationship between the in vivo fractional synthesis of triglycerides ( f TG ) in subcutaneous (s.q.) abdominal adipose tissue (AT), HDL-C concentrations and HDL particle size composition in non-diabetic humans. Methods: The f TG in s.q. abdominal AT was measured in 16 non-diabetic volunteers (7 women, 9 men; Age: 49 ± 20 years; BMI: 31 ± 5 kg/m; Fasting Plasma Glucose: 90 ± 10 mg/dl) after 2 H2 O labeling. HDL-C concentration and subclasses, large (L-HDL), intermediate (I-HDL) and small (S-HDL) were measured. Results: Linear regression analyses demonstrated significant associations of f TG with plasma concentration of HDL-C ( r = 0.625, p = 0.009) and percent contribution of L-HDL ( r = 0.798, p < 0.001), I-HDL ( r = −0.765, p < 0.001) and S-HDL ( r = −0.629, p = 0.009). When analyses were performed by gender, the associations remained significant in women (HDL-C: r = 0.822, p = 0.023; L-HDL: r = 0.892, p = 0.007; I-HDL: r = −0.927, p = 0.003) but not men. Conclusions: Our study demonstrated an in vivo association between subcutaneous abdominal adipose tissue lipid dynamics and HDL parameters in humans, but this was true for women not men. Positive association with L-HDL and negative with I-HDL suggest that subcutaneous abdominal adipose tissue lipid dynamics may play an important role in production of mature functional HDL particles. Further studies evaluating the mechanism responsible for these associations and the observed gender differences are important and warranted to identify potential novel targets of intervention to increase the production of atheroprotective subclasses of HDL-Cs and thus decreasing the risks of development of atherosclerotic conditions. Highlights: Adipose tissue (AT) lipid dynamics associates with HDL-C metabolism in humans. However this association is gender-specific. AT triglyceride synthesis directly associates with large HDL-C particles. AT triglyceride synthesis inversely associates with intermediate HDL-C particles. Modulation of AT lipid dynamics may help prevent the development of atherosclerosis.