A commercial PCV2a-based vaccine significantly reduces PCV2b transmission in experimental conditions. Issue 33 (19th July 2016)

• Record Type: Journal Article
• Authors: Rose, N.; Andraud, M.; Bigault, L.; Jestin, A.; Grasland, B.
• Is Part Of: Vaccine. Volume 34:Issue 33(2016)
• Page Start: 3738
• Page End: 3745
• Publication Date: 2016-07-19
• Subjects: Porcine Circovirus type 2 -- Vaccination -- Transmission -- Reproduction number; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2016.06.005
• Languages: English
• ISSNs: 0264-410X

Abstract

Abstract: Transmission characteristics of PCV2 have been compared between vaccinated and non-vaccinated pigs in experimental conditions. Twenty-four Specific Pathogen Free (SPF) piglets, vaccinated against PCV2 at 3 weeks of age (PCV2a recombinant CAP protein-based vaccine), were inoculated at 15 days post-vaccination with a PCV2b inoculum (6 ⋅ 10 5 TCID50 ), and put in contact with 24 vaccinated SPF piglets during 42 days post-inoculation. Those piglets were shared in six replicates of a contact trial involving 4 inoculated piglets mingled with 4 susceptible SPF piglets. Two replicates of a similar contact trial were made with non-vaccinated pigs. Non vaccinated animals received a placebo at vaccination time and were inoculated the same way and at the same time as the vaccinated group. All the animals were monitored twice weekly using quantitative real-time PCR and ELISA for serology until 42 days post-inoculation. The frequency of infection and the PCV2 genome load in sera of the vaccinated pigs were significantly reduced compared to the non-vaccinated animals. The duration of infectiousness was significantly different between vaccinated and non-vaccinated groups (16.6 days [14.7;18.4] and 26.6 days [22.9;30.4] respectively). The transmission rate was also considerably decreased in vaccinated pigs ( β = 0.09 [0.05–0.14] compared to β = 0.19 [0.11–0.32] in non-vaccinated pigs). This led to an estimated reproduction ratio of 1.5 [95% CI 0.8 – 2.2] in vaccinated animals versus 5.1 [95% CI 2.5 – 8.2] in non-vaccinated pigs when merging data of this experiment with previous trials carried out in same conditions.