Effects of cryotherapy combined with therapeutic ultrasound on oxidative stress and tissue damage after musculoskeletal contusion in rats. Issue 4 (December 2016)

• Record Type: Journal Article
• Authors: Martins, C.N.; Moraes, M.B.; Hauck, M.; Guerreiro, L.F.; Rossato, D.D.; Varela, A.S.; da Rosa, C.E.; Signori, L.U.
• Is Part Of: Physiotherapy. Volume 102:Issue 4(2016)
• Page Start: 377
• Page End: 383
• Publication Date: 2016-12
• Subjects: Rehabilitation -- Skeletal muscle -- Inflammation -- Physical therapy modalities -- Oxidative stress -- Ultrasonic therapy; Physical therapy -- Periodicals; Therapeutics, Physiological -- Periodicals; 615.8205
• DOI: 10.1016/j.physio.2015.10.013
• Languages: English
• ISSNs: 0031-9406

Abstract

Abstract: Objective: To investigate the combined effects of cryotherapy and pulsed ultrasound therapy (PUT) on oxidative stress parameters, tissue damage markers and systemic inflammation after musculoskeletal injury. Design: Experimental animal study. Setting: Research laboratory. Participants: Seventy male Wistar rats were divided into five groups: control, lesion, cryotherapy, PUT, and cryotherapy + PUT. Interventions: The gastrocnemius muscle was injured by mechanical crushing. Cryotherapy was applied immediately after injury (immersion in water at 10 °C for 20 minutes). PUT was commenced 24 hours after injury (1 MHz, 0.4 W/cm 2SPTA, 20% duty cycle, 5 minutes). All animals were treated every 8 hours for 3 days. Main outcome measures: Oxidative stress in muscle was evaluated by concentration of reactive oxygen species (ROS), lipid peroxidation (LPO), anti-oxidant capacity against peroxyl radicals (ACAP) and catalase. Plasma levels of creatine kinase (CK), lactate dehydrogenase (LDH) and C-reactive protein (CRP) were assessed. Results: When applied individually, cryotherapy and PUT reduced CK, LDH, CRP and LPO caused by muscle damage. Cryotherapy + PUT in combination maintained the previous results, caused a reduction in ROS [ P = 0.005, mean difference −0.9 × 10 −8 relative area, 95% confidence interval (CI) −0.2 to −1.9], and increased ACAP { P = 0.007, mean difference 0.34 1/[relative area with/without 2, 2-azobis(2-methylpropionamidine)dihydrochloride], 95% CI 0.07 to 0.61} and catalase ( P = 0.002, mean difference 0.41 units/mg protein, 95% CI 0.09 to 0.73) compared with the lesion group. Conclusions: Cryotherapy + PUT in combination reduced oxidative stress in muscle, contributing to a reduction in adjacent damage and tissue repair.