Screening and characterizing a denitrifying phosphorus-accumulating bacterium isolated from a circular plug-flow reactor. Issue 22 (16th November 2016)

• Record Type: Journal Article
• Authors: Xie, En; Ding, Aizhong; Zheng, Lei; Dou, Junfeng; Anderson, Bruce; Huang, Xiaolong; Jing, Ruoting
• Is Part Of: Environmental technology. Volume 37:Issue 22(2016)
• Page Start: 2823
• Page End: 2829
• Publication Date: 2016-11-16
• Subjects: 16S rRNA -- denitrification -- phosphorus -- Pseudomonas sp -- poly-β-hydroxybutyrate; Environmental engineering -- Periodicals; Environmental protection -- Periodicals; 628.05
• DOI: 10.1080/09593330.2016.1167247
• Languages: English
• ISSNs: 0959-3330

Abstract

ABSTRACT: Denitrifying phosphorus-accumulating organisms (DNPAO) are viewed as one of the most effective means to solve the removal contradiction of nitrogen and phosphorus in wastewater treatment. In this study, we isolated a DNPAO (C-17, accession number: KU745702) from activated sludge in a patented circular plug-flow reactor, physiologically to Pseudomonas sp. based on 16S rRNA sequence and phenotypic characteristics. The results of denitrifying phosphorus-accumulating experiment showed that Pseudomonas C-17 has high removal efficiencies for and NO3 –N, 75% and 87%, respectively. The ratio of phosphorus release was 25.0 mg (with anabolism) and 26.8 mg (without anabolism), respectively. Our results indicated that Pseudomonas C-17 had strong capacity of phosphorus release, and its uptake is often imprecisely evaluated by ignoring the part of metabolic consumption. Pseudomonas C-17 is capable of utilizing oxygen, nitrate and nitrite as electron acceptors under experimental conditions.