Allyl isothiocyanate suppresses the proteolytic activation of sterol regulatory element-binding proteins and de novo fatty acid and cholesterol synthesis*. Issue 5 (3rd May 2016)

• Record Type: Journal Article
• Authors: Miyata, Shingo; Inoue, Jun; Shimizu, Makoto; Sato, Ryuichiro
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 5(2016)
• Page Start: 1006
• Page End: 1011
• Publication Date: 2016-05-03
• Subjects: allyl isothiocyanate -- cholesterol -- fatty acid -- SREBP; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2015.1132154
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract : Sterol regulatory element-binding proteins (SREBPs) are a family of transcription factors that regulate lipid homeostasis by controlling the expression of genes involved in fatty acid and cholesterol synthesis. In this study, we used a stable cell line that expresses a luciferase reporter gene driven by an SRE-containing fatty acid synthase promoter to identify allyl isothiocyanate (AITC), one of the major isothiocyanates in cruciferous vegetables, as a novel SREBP inactivator. We found that AITC downregulated the proteolytic processing of SREBPs and the expression of their target genes in human hepatoma Huh-7 cells. Furthermore, AITC reduced the de novo synthesis of both fatty acids and cholesterol. Our results indicate a novel physiological function of AITC in lipid metabolism regulation. Graphical abstract: Allyl isothiocyanate suppresses the processing of SREBP-1 and SREBP-2.