Combination of 3D Extruded-based Poly (ɛ-caprolactone) Scaffolds with Mesenchymal Stem/Stromal Cells: Strategy Optimization. (2015)

• Record Type: Journal Article
• Authors: Moura, Carla Sofia; Silva, Cláudia Lobato da; Bártolo, Paulo Jorge; Ferreira, Frederico Castelo
• Is Part Of: Procedia engineering. Volume 110(2015)
• Page Start: 122
• Page End: 127
• Publication Date: 2015
• Subjects: Mesenchymal Stem/Stromal Cells -- Poly (ɛ-caprolactone) -- Scaffolds -- Tissue Engineering.; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Conference proceedings; Periodicals; 620.005
• DOI: 10.1016/j.proeng.2015.07.019
• Languages: English
• ISSNs: 1877-7058

Abstract

Abstract: This study uses a combination of 3D extruded-based biodegradable scaffolds and human bone marrow-derived mesenchymal stem/stromal cells to understand how scaffold properties can contribute for efficient cell seeding, proliferation and migration. Highly interconnected porous poly(ɛ-caprolactone) scaffolds were produced by a layer-by-layer extrusion. Two configurations were studied with larger (390 μm) or smaller pore size (190 μm) at the centre. MSC, seeded on the top centre of the scaffold surface, increased 5 folds after 13 days of cultivation for both configurations, allowing us to conclude that in this range of pore sizes pose no limitations for cell migration are observed.