Differential proteomic analysis of outer membrane enriched extracts of Bacteroides fragilis grown under bile salts stress. (June 2016)

• Record Type: Journal Article
• Authors: Boente, Renata F.; Pauer, Heidi; Silva, Deborah N.S.; Filho, Joaquim Santos; Sandim, Vanessa; Antunes, Luis Caetano M.; Ferreira, Rosana Barreto Rocha; Zingali, Russolina B.; Domingues, Regina M.C.P.; Lobo, Leandro A.
• Is Part Of: Anaerobe. Volume 39(2016)
• Page Start: 84
• Page End: 90
• Publication Date: 2016-06
• Subjects: Bacteroides fragilis -- Resistance -- Bile salts -- ESI-Q/TOF -- Outer membrane proteins; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2016.03.003
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: Bacteroides fragilis is the most commonly isolated anaerobic bacteria from infectious processes. Several virulence traits contribute to the pathogenic nature of this bacterium, including the ability to tolerate the high concentrations of bile found in the gastrointestinal tract (GIT). The activity of bile salts is similar to detergents and may lead to membrane permeabilization and cell death. Modulation of outer membrane proteins (OMPs) is considered a crucial event to bile salts resistance. The primary objective of the current work was to identify B. fragilis proteins associated with the stress induced by high concentration of bile salts. The outer membrane of B. fragilis strain 638R was isolated after growth either in the presence of 2% conjugated bile salts or without bile salts. The membrane fractions were separated on SDS-PAGE and analyzed by ESI-Q/TOF tandem mass spectrometry. A total of 37 proteins were identified; among them nine were found to be expressed exclusively in the absence of bile salts whereas eight proteins were expressed only in the presence of bile salts. These proteins are related to cellular functions such as transport through membrane, nutrient uptake, and protein-protein interactions. This study demonstrates the alteration of OMPs composition in B. fragilis during bile salts stress resistance and adaptation to environmental changes. Proteomics of OMPs was also shown to be a useful approach in the identification of new targets for functional analyses. Highlights: We identified OMPs differentially expressed in response to bile in Bacteroides fragilis . Among 37 proteins identified, eight proteins were detected only in presence of bile. Their function includes nutrient uptake, cell maintenance and transport. Proteins acting as secretins and lipocalin were also found only in presence of bile. Our results showed B. fragilis modulates its surface in response to bile salts.