A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters. (June 2016)

• Record Type: Journal Article
• Authors: Bühligen, Franziska; Lucas, Rico; Nikolausz, Marcell; Kleinsteuber, Sabine
• Is Part Of: Anaerobe. Volume 39(2016)
• Page Start: 114
• Page End: 116
• Publication Date: 2016-06
• Subjects: mcrA -- Community fingerprinting -- BstNI -- MwoI -- Methanogenic archaea -- Biogas; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2016.03.013
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing. Highlights: A database for T-RFLP profiling of methanogens in anaerobic digesters is presented. T-RF values of mcrA amplicons can be assigned at least to the family level. Methanogens in biogas reactors can be identified without cloning and sequencing.