"Dilute-and-Shoot" Rapid-Separation Liquid Chromatography Tandem Mass Spectrometry Method for Fast Detection of Thiodiglycolic Acid in Urine. Issue 5 (October 2015)

• Record Type: Journal Article
• Authors: Baygildiev, Timur; Braun, Arcady; Stavrianidi, Andrey; Rodin, Igor; Shpigun, Oleg; Rybalchenko, Igor; Ananieva, Irina
• Is Part Of: European journal of mass spectrometry. Volume 21:Issue 5(2015)
• Page Start: 733
• Page End: 738
• Publication Date: 2015-10
• Subjects: dilute-and-shoot -- tandem mass spectrometry -- chemical warfare agents -- sulfur mustard -- thiodiglycolic acid; Mass spectrometry -- Periodicals; Mass Spectrometry; Mass spectrometry; Periodicals; Periodicals; 543.6505
• DOI: 10.1255/ejms.1387
• Languages: English
• ISSNs: 1469-0667

Abstract

A new sensitive rapid-separation liquid chromatography tandem mass spectrometry approach for the determination of thiodiglycolic acid (TDGA) in urine has been developed. The use of the "dilute-and-shoot" method helps to shorten the sample preparation stage and provides a sensitive and direct approach for TDGA determination in urine. Chromatographic separation of the analyte and other urine compounds was achieved using a reverse-phase liquid chromatography column with mobile phases consisting of 0.1% formic acid in water and acetonitrile in a gradient elution mode. For the identification and quantification of TDGA electrospray ionization-tandem mass spectrometry monitoring, two precursor-to-product ion transitions were used. The method demonstrates good linearity and has a detection limit of 50 ng mL −1 in urine.