Letter: Observation of the 16O/18O Exchange during Electrospray Ionization. Issue 2 (April 2015)

• Record Type: Journal Article
• Authors: Kostyukevich, Yury; Kononikhin, Alexey; Popov, Igor; Nikolaev, Eugene
• Is Part Of: European journal of mass spectrometry. Volume 21:Issue 2(2015)
• Page Start: 109
• Page End: 113
• Publication Date: 2015-04
• Subjects: isotopic exchange -- mass spectrometry -- ESI -- FT ICR; Mass spectrometry -- Periodicals; Mass Spectrometry; Mass spectrometry; Periodicals; Periodicals; 543.6505
• DOI: 10.1255/ejms.1373
• Languages: English
• ISSNs: 1469-0667

Abstract

Isotopic exchange approach coupled to high-resolution mass spectrometry has become the power analytical approach for a wide range of analytical and bioanalytical applications. Considerable efforts have been dedicated to developing fast exchange techniques directly in the ionization source. But all such methods are limited to the hydrogen/deuterium exchange approaches. In this paper we demonstrate that certain types of oxygen atoms can also be exchanged for 18 O on the time scale of the ionization process. Using HIO3 and NaIO4 and by infusing the heavy water H2 18 O in the ESI source we have demonstrated that it is possible to obtain a high level of oxygen exchange. It was observed that the rate of this exchange depends to a large extent on the temperature of the desolvating capillary of the mass spectrometer. Several other species, such as peptides, oligonucleotides and low weight organic molecules, were subjected to in-ESI 16 O/ 18 O exchange but the exchange was not observed.