Efficient hydrolysis of corncob residue through cellulolytic enzymes from Trichoderma strain G26 and l-lactic acid preparation with the hydrolysate. (October 2015)

• Record Type: Journal Article
• Authors: Xie, Lulu; Zhao, Jin; Wu, Jian; Gao, Mingfu; Zhao, Zhewei; Lei, Xiangyun; Zhao, Yi; Yang, Wei; Gao, Xiaoxue; Ma, Cuiyun; Liu, Huanfei; Wu, Fengjuan; Wang, Xingxing; Zhang, Fengwei; Guo, Pengyuan; Dai, Guifu
• Is Part Of: Bioresource technology. Volume 193(2015)
• Page Start: 331
• Page End: 336
• Publication Date: 2015-10
• Subjects: Corncob residue -- Solid-state fermentation -- Saccharification -- Trichoderma longibrachiatum -- Cellulase; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.06.101
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Trichoderma longibrachiatum G26 can produce high levels of cellulolytic enzymes. The cellulolytic enzymes can overcome the product inhibition. A three-stage enzymatic saccharification strategy for corncob residue is proposed. The hydrolysate of corncob residue is suitable forl -lactic acid fermentation. Abstract: To prepare fermentable hydrolysate from corncob residue (CCR), Trichoderma strain G26 was cultured on medium containing CCR for production of cellulolytic enzymes through solid-state fermentation (SSF), resulting in 71.3 IU/g (FPA), 136.2 IU/g (CMCase), 85.1 IU/g (β-glucosidase) and 11, 344 IU/g (xylanase), respectively. Through a three-stage saccharification strategy, CCR was hydrolyzed by the enzymatic solution (6.5 FPU/ml) into fermentable hydrolysate containing 60.1 g/l glucose (81.2% cellulose was converted at solid loading of 12.5%), 21.4% higher than that by the one-stage method. And then the hydrolysate was used to producel -lactic acid by a previous screened strain Bacillus coagulans ZX25 in the submerged fermentation. 52.0 g/ll -lactic acid was obtained after fermentation for 44 h, with 86.5% glucose being converted tol -lactic acid. The results indicate that the strains and the hydrolysis strategy are promising for commercial production ofl -lactic acid from CCR and other biomass.