Impact of the substrate loading regime and phosphoric acid supplementation on performance of biogas reactors and microbial community dynamics during anaerobic digestion of chicken wastes. (October 2015)

• Record Type: Journal Article
• Authors: Belostotskiy, Dmitry E.; Ziganshina, Elvira E.; Siniagina, Maria; Boulygina, Eugenia A.; Miluykov, Vasili A.; Ziganshin, Ayrat M.
• Is Part Of: Bioresource technology. Volume 193(2015)
• Page Start: 42
• Page End: 52
• Publication Date: 2015-10
• Subjects: Biogas -- Chicken wastes -- Phosphoric acid -- Ammonia inhibition -- Microbial diversity; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.06.066
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Anaerobic digestion of chicken wastes under various conditions was investigated. Phosphoric acid at moderate level positively affected the anaerobic digestion process. 454 pyrosequencing approach was used to evaluate the microbial community diversity. Bacteroidales, Erysipelotrichaceae, Clostridium, Methanosarcina were the abundant taxa. The major process parameter shaping community structure was the high ammonia level. Abstract: This study evaluates the effects of increasing organic loading rate (OLR) and decreasing hydraulic retention time (HRT) as well as phosphoric acid addition on mesophilic reactors' performance and biogas production from chicken wastes. Furthermore, microbial community composition in reactors was characterized by a 16S rRNA gene-based pyrosequencing analysis. Each step of increasing OLR impacted on the activity of microorganisms what caused a temporary decrease in biogas production. The addition of phosphoric acid resulted in the increased biogas production with values between 361 and 447 mL gVS −1 from day 61 to day 74 compared to control reactor (309–350 mL gVS −1 ). With reactors' operation, Bacteroidetes phylotypes were noticeably replaced with Firmicutes representatives, and significant increase of Clostridium sp. was identified. Within Euryarchaeota, Methanosarcina sp. dominated in all analyzed samples, in which high ammonium levels were detected (3.4–4.9 NH4 + -N g L −1 ). These results can help in better understanding the anaerobic digestion process of simultaneously ammonium/phosphate-rich substrates.